BORON CONCENTRATION AFFECTS IN VITRO POLLEN GERMINATION AND TUBE GROWTH IN KIWIFRUIT (ACTINIDIA DELICIOSA) CULTIVAR 'MATUA'

C. Sotomayor, R. Ruiz, J. García-Huidobro
The effect of varying boron (B) concentrations on in vitro pollen germination and tube growth of ‘Matua’ kiwifruit was evaluated. Five concentrations of boric acid (H3BO3): 0, 25, 50, 100 and 500 mg L-1, were added to the germination media, prepared with 5% sucrose and 2% agar. ‘Matua’ pollen was placed on the agar media and incubated in Petri dishes at 20°C for 48 h, with germination evaluated after 24 h. Pollen incubated at concentrations of 25 and 50 mg L-1 boric acid showed 67.5 and 73.5% of pollen germination, respectively. Significant reductions in pollen germination, 24.0 and 22.7%, were observed at B concentrations of 100 and 500 mg L-1, respectively. In the media prepared without B, only 16.7% germination was observed. After 48 h of incubation, in media prepared with 25 and 50 mg L-1 of boric acid, pollen tube lengths reached 53.2 and 66.7 µm, respectively, being significantly longer than those incubated in 100 and 500 mg L-1 B media (27.2 and 22.4 µm, respectively), and the 0 mg L-1 B media, where pollen tube length reached only 18.3 µm. It can be concluded that boric acid concentrations 100 mg L-1 or higher in the growing media reduce ‘Matua’ pollen germination and tube growth.
Sotomayor, C., Ruiz, R. and García-Huidobro, J. (2012). BORON CONCENTRATION AFFECTS IN VITRO POLLEN GERMINATION AND TUBE GROWTH IN KIWIFRUIT (ACTINIDIA DELICIOSA) CULTIVAR 'MATUA'. Acta Hortic. 932, 517-521
DOI: 10.17660/ActaHortic.2012.932.75
https://doi.org/10.17660/ActaHortic.2012.932.75
pollen tube growth, boric acid, growing media, seed number
English

Acta Horticulturae