EFFECTS OF GAMMA-RAY IRRADIATION ON AFRICAN VIOLET IN VITRO ADVENTITIOUS SHOOTS
The effects of 1.25 MeV gamma-ray irradiation on Saintpaulia ionantha adventitious shoots derived from in vitro leaf explants were studied with regard to fresh weight, green leaf production, malformed leaves and rate of mortality. Leaf explants were cultured on basal MS medium supplemented with NAA (0.2 mg L-1), BAP (1 mg L-1) and were irradiated with 0, 10, 20, 30 and 40 Gy of gamma-ray after 3 weeks of incubation. Data were recorded after 6 weeks of irradiation. Results revealed that higher intensities of gamma-ray caused the greater rate of mortality and reduction in green leaf production and also, malformed leaves. Also, it was shown that different treatments of gamma-ray had significant effects on the studied traits. It was observed that mortality started at 30 Gy (22.92%) and reached 50% at 40 Gy as LD50. So, we recommend the 40 Gy irradiation for selecting tolerant varieties of African Violet. The regenerated shoots were rooted in mixed medium include perlite and coco peat (1:1) in greenhouse conditions.
Farjadi-Shakib, M., Naderi , R. and Mousavi , A. (2012). EFFECTS OF GAMMA-RAY IRRADIATION ON AFRICAN VIOLET IN VITRO ADVENTITIOUS SHOOTS . Acta Hortic. 937, 923-927
chimera, mutation, Saintpaulia ionantha, tissue culture