PRONAMIDE-INDUCED POLYPLOIDY IN RHYNCHOSTYLIS AND DENDROBIUM

P. Tantasawat, A. Khairum, W. Chaowiset , A. Wannajindaporn
The effects of pronamide on in vitro germinated seeds of Rhynchostylis gigantean were evaluated using a range of pronamide concentrations (0, 100, 200, and 400 μM) at various exposure times (4 and 8 d). Polyploid plants could be identified based on leaf thickening, leaf length/width ratio, size and density of stomata. Pronamide treatments at 100 and 200 μM were found effective to produce polyploid plants. In addition, pronamide was also effective for the induction of polyploidy in protocorm explants of Dendrobium at lower concentrations (10 and 100 μM) and a reduced exposure time (2 d). These results suggest pronamide may be a valuable tool in the production of polyploid orchids in the future breeding programs.
Tantasawat, P., Khairum, A., Chaowiset , W. and Wannajindaporn, A. (2012). PRONAMIDE-INDUCED POLYPLOIDY IN RHYNCHOSTYLIS AND DENDROBIUM. Acta Hortic. 937, 615-620
DOI: 10.17660/ActaHortic.2012.937.75
https://doi.org/10.17660/ActaHortic.2012.937.75
double chromosome, mutagenesis, orchid, tissue culture
English

Acta Horticulturae