MICROPROPAGATION OF TREE PEONY FROM LATERAL BUDS
Tree peony Wu Long Peng Sheng was micropropagated using lateral buds as explants. It was the first time to study the effect of cutting lateral buds on the adventitious buds differentiation. The influence of the cutting on the adventitious buds induction, the multiplication and rooting were also studied. The results were as follows: (1) After lateral bud cutting, the number of adventitious buds increased significantly with the maximum reaching 96%, from 2.6 times to 4.8 times of the controls.(2) The best medium for adventitious buds multiplication was WPM + 6-BA 3.0 mg/L + IAA 0.2 mg/L, with the multiplication rate up to 7.9 times of the control. (3) The rooting rate of adventitious buds was up to 81.3% on half-strength WPM medium supplemented with NAA 1.0 mg/L and IBA 4.0 mg/L. The plantlets were successfully transplanted to pots and the survival rate of transplanting of plantlets was 90%.
Jia, W.-Q., Liu, H.-C., Du, X.-H. and Zhao, Y.-P. (2012). MICROPROPAGATION OF TREE PEONY FROM LATERAL BUDS . Acta Hortic. 937, 635-640
micropropagation, cutting, proliferation, rooting