MICROSCOPIC STUDY OF ARTICHOKE (CYNARA SCOLYMUS L.) FLORAL PIECES IN ORDER TO DEVELOP A HAPLOID PRODUCTION TECHNIQUE
Haplomethods are nowadays commonly used for several agronomic plants, and allow the fast production of perfectly homozygous individuals. For artichoke, the selection of interesting recessive agronomic traits would be of great interest. In this work, we tested the possibility of obtaining haploids in artichokes either by gynogenesis or androgenesis, two previously tested techniques. Using protocols, successful for gerbera and sunflower, we obtained structures after in vitro plating of unpollinated flowers but, microscopic observations showed that they probably resulted from embryo sac abortion. We were able to reproduce previous results obtained for culture of isolated microspores, with a development stopped after the first division of the pro-embryo. The microscopic techniques described are now used for the characterisation of different behaviours of cells, according to culture conditions. This work allowed a better understanding of tissue development in vitro needed for further research to produce di-haploids of artichokes.
Guerrand, J., Euzen, M., Courand , D., Bodin, M., Tanguy , J.L. and Ménard, V. (2012). MICROSCOPIC STUDY OF ARTICHOKE (CYNARA SCOLYMUS L.) FLORAL PIECES IN ORDER TO DEVELOP A HAPLOID PRODUCTION TECHNIQUE. Acta Hortic. 942, 165-170
haplomethod, tissue culture, breeding, microspore, ovule