GENETIC RELATEDNESS AMONG OLEA EUROPAEA L. CULTIVARS ESTIMATED BY RAPD ANALYSIS

S. Gomes, P. Martins-Lopes, J. Lopes, H. Guedes-Pinto
Random Amplified Polymorphic DNA (RAPDs) analysis was used to evaluate the genetic diversity and variability present in 41 olive cultivars grown in Trás-os-Montes Region. Eleven RAPD primers were used. After agarose gels analysis, RAPDs banding patterns were transformed into binary data of presence-absence and matrices were processed with NTSYS-PC. Genetic distances between all pair wise combinations of the cultivars were calculated using Jaccard’s coefficient of similarity and dendrograms were constructed by the UPGMA method. All primers produced polymorphic bands. A total of 114 reproducible RAPD bands were observed, of which 100 were polymorphic, accounting for a high percentage (86%) of polymorphism. Five unique bands, able to be converted into Sequence Characterized Amplified Region (SCAR) markers, were obtained. Dendrogram similarity relationships revealed some clustering according to the olive cultivars geographical origin.
Gomes, S., Martins-Lopes, P., Lopes, J. and Guedes-Pinto, H. (2012). GENETIC RELATEDNESS AMONG OLEA EUROPAEA L. CULTIVARS ESTIMATED BY RAPD ANALYSIS. Acta Hortic. 949, 61-66
DOI: 10.17660/ActaHortic.2012.949.7
https://doi.org/10.17660/ActaHortic.2012.949.7
genetic variability, RAPD molecular markers, Olea, genetic relatedness
English

Acta Horticulturae