SOME PROPERTIES OF A NUCLEIC ACID ASSOCIATED WITH LITTLE CHERRY DISEASE

R.I. HAMILTON, J.A. DODDS, J. RAINE
A nucleic acid was isolated from leaves of sweet cherry (Prunus avium L.) infected with little cherry disease (LCD) by a method which is highly selective for double-stranded RNA (ds-RNA). The nucleic acid was susceptible to RNase in low salt but not in high salt and it was resistant to RNase-free DNase. Its molecular weight, estimated by polyacrylamide gel electrophoresis using the ds-RNA of tissue infected with turnip yellow mosaic virus, as a standard, was slightly greater than 4X106. The ds-RNA was not obtained from sweet cherry known to be free of LCD nor from sweet cherry infected with known viruses.
HAMILTON, R.I., DODDS, J.A. and RAINE, J. (1981). SOME PROPERTIES OF A NUCLEIC ACID ASSOCIATED WITH LITTLE CHERRY DISEASE. Acta Hortic. 94, 75-78
DOI: 10.17660/ActaHortic.1981.94.6
https://doi.org/10.17660/ActaHortic.1981.94.6

Acta Horticulturae