PROTOCOL DEVELOPMENT FOR IN VITRO ASSESSMENT OF CADMIUM TOLERANCE IN BLACK ALDER AND BASKET WILLOW AT THE CALLUS AND WHOLE PLANT LEVELS
This study was carried out in the framework of an INTERREG IV-A Greater Region project (ECOLIRIMED). We initially optimised in vitro culture conditions for calli and plants. Calli were induced from stem internodes (black alder) or leaves (basket willow) of in vitro plants on MS medium supplemented with 1 µM 2,4-D and 1 µM BAP (alder) or with 1 µM 2,4-D only (willow). Plant cultures were established from shoot apices of in vitro plants. In the case of alder, plants were grown on WPM with either 1 µM BAP (shoot proliferation) or 2.5 µM IBA (shoot rooting). WPM enrichment with 300 µM Fe-EDDHA and the use of 3% glucose in aerated jars have proven beneficial for alder plant development. Regarding willow, both shoot proliferation and rooting occur in aerated jars on MS medium supplemented with 2% sucrose and 0.5 µM IBA. Once the culture conditions optimised, the effect of cadmium (Cd) (calli: 0-500 µM, plants: 0-200 µM) on growth and Cd accumulation pattern was investigated. At the callus level, growth decreased with increasing Cd concentration in the medium, the effect being significant at 100 µM in alder and at 400 µM in willow. At the whole plant level, however, growth inhibition was recorded at 150 µM Cd for both species. Among the plant parts, roots of alder and stems and leaves of willow were the most affected in the presence of Cd. In calli, Cd accumulated with increasing Cd concentration in the medium, the accumulation being larger in alder than in willow. In plants, Cd also accumulated with the extent of treatment in the different plant parts of the two species. In alder plants, Cd was mainly accumulated in roots (roots>stems>leaves). In willow plants, however, Cd was almost equally distributed among the different plant parts.
Bajji, M. and Druart, PH. (2012). PROTOCOL DEVELOPMENT FOR IN VITRO ASSESSMENT OF CADMIUM TOLERANCE IN BLACK ALDER AND BASKET WILLOW AT THE CALLUS AND WHOLE PLANT LEVELS. Acta Hortic. 961, 123-131
Alnus glutinosa, Salix viminalis, woody plants, micropropagation, heavy metals, phytoremediation