IN VITRO PICLORAM-INDUCED SOMATIC EMBRYOGENESIS FROM LEAFLET OF PRUNUS INCISE AND EXPRESSION PROFILE OF CDC2 GENE
A procedure for inducing somatic embryogenesis from leaflets has been developed with Prunus incise, using picloram enrichment of MS basal medium combined to darkness exposure as culture treatments and 0.4 µM BAP (6-benzylaminopurine) and 0.05 µM NAA (α-naphthalene acetic acid) with daylight exposure for expression. In this study, we show that embryogenesis was differently affected by the picloram concentration (2 to 6 µM) and the treatment duration (10 to 40 days). The best rate of embryogenic leaves (27%) was obtained after 30 days on MS medium supplemented with 4 µM picloram. According to the original position of the leaves on the shoot, the second expanded leaf from the apex was the most responsive. In parallel, a molecular study was conducted in order to provide greater insight in to the biological background of the regeneration pathway of this woody species. The cDNA of a cdc2 gene involved in cell cycle regulation was partially cloned and sequenced. Differential transcript accumulations from that gene have been recorded in leaves and their originating callus tissues according to the culture period. The possible involvement of the Picdc2 in the crucial steps of the embryogenic pathway is discussed, in regard of one of the main enzymes of the cell cycle regulation machinery, but also as a marker of specific cell division competent stage in non-dividing cells.
Ben Mahmoud, K., Delporte, F., Muhovski, Y., Elloumi, N., Jemmali, A. and Druart, PH. (2012). IN VITRO PICLORAM-INDUCED SOMATIC EMBRYOGENESIS FROM LEAFLET OF PRUNUS INCISE AND EXPRESSION PROFILE OF CDC2 GENE. Acta Hortic. 961, 133-137
dedifferentiation, cherry tree, cell cycle, gene expression, auxin