RETROTRANSPOSITION EVENTS IN BARLEY CALLUS CULTURES

A. Temel, N. Gozukirmizi
Gene transfer techniques offer an alternative to improve existing cultivars and to obtain new ones. Establishment of tissue culture procedures is a prerequisite in order to obtain stable transformants. However, plants regenerated from tissue culture may exhibit somaclonal variation. These variations, generally undesired, include nucleotide mutations, gene activation/silencing, chromosome abnormalities. Gene silencing and activation occur by DNA methylation and activation of transposable elements. Movement of transposable elements also causes insertional polymorphisms in genome. DNA polymorphisms in 15-day-old, 30-day-old calli and 4-day-old barley (Hordeum vulgare ‘Zafer-160’) seedling were investigated with Inter-Retrotransposon Amplified Polymorphism (IRAP), a retrotransposon-based marker system. PCR primers designed from BARE-1 and Sukkula elements were combined and amplification products were resolved on non-denaturing polyacrylamide gels. Different polymorphic bands were detected, although banding patterns were highly monomorphic among different tissues. However, control (4-day-old seedling) tissue and 30-day-old callus gave more similar fingerprints.
Temel, A. and Gozukirmizi, N. (2012). RETROTRANSPOSITION EVENTS IN BARLEY CALLUS CULTURES. Acta Hortic. 961, 175-179
DOI: 10.17660/ActaHortic.2012.961.20
https://doi.org/10.17660/ActaHortic.2012.961.20
retrotransposon, IRAP, BARE-1, Sukkula, polymorphism
English

Acta Horticulturae