GENETIC STABILITY IN MICRO-CLONES OF 'WOOD-APPLE' DERIVED FROM DIFFERENT PATHWAYS OF MICROPROPAGATION AS REVEALED BY RAPD AND ISSR MARKERS

P. Joshi, S.D. Purohit
Micropropagation of Feronia limonia, a medicinally important plant belonging to the family Rutaceae was achieved using two different pathways involving enhanced axillary branching and callus organogenesis. Shoot cultures derived from them were sub-cultured up to ten passages and micro-clones were subjected to test of fidelity using RAPD and ISSRs markers. Of 78 primers (53 RAPD + 25 ISSRs), 35 primers (27 RAPD + 8 ISSRs) produced clear, distinct and scorable bands with an average of 8.1 bands per primer. Molecular analysis of the data revealed that micro-clones derived from mature node explants showed no variability while clones derived from cotyledonary node explants showed two percent variability. Micro-propagules obtained from callus organogenesis pathway showed six percent variability. Screening of micro-clones revealed that true-to-type plantlets could be obtained only through enhanced axillary branching using mature node segments.
Joshi, P. and Purohit, S.D. (2012). GENETIC STABILITY IN MICRO-CLONES OF 'WOOD-APPLE' DERIVED FROM DIFFERENT PATHWAYS OF MICROPROPAGATION AS REVEALED BY RAPD AND ISSR MARKERS. Acta Hortic. 961, 217-224
DOI: 10.17660/ActaHortic.2012.961.26
https://doi.org/10.17660/ActaHortic.2012.961.26
molecular markers, genetic fidelity, Feronia limonia, micropropagation, axillary branching, callus organogenesis, medicinal plant
English

Acta Horticulturae