CALLUS INDUCTION OF STEMONA CURTISII HOOK. F.
A successful protocol was established for callus induction and regeneration of Stemona curtisii Hook. f. The sterilized seeds were cultured on Murashige and Skoog (MS, 1962) supplemented with 0-0.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) in dark and light conditions. The combination of 0-2 mg L-1 of 2,4-D and benzyladenine (BA) or thidiazuron (TDZ) was also examined in seed and shoot culture. The results show that callus induction was highest from seed. The 0.4 mg L-1 2,4-D in the dark condition treatment was suitable to promoted callus formation at 62.50%. The combination of 2,4-D with BA or TDZ strongly induced callus formation (2 mg L-1 BA with 2 mg L-1 2,4-D). Plant regeneration from callus was successfully induced via both organogenesis and embryogenesis.
Montri, N., Chaisriha, S. and Suwanphakdee, C. (2012). CALLUS INDUCTION OF STEMONA CURTISII HOOK. F.. Acta Hortic. 961, 273-278
Stemonaceae, tissue culture