MATURATION OF OLIVE SOMATIC EMBRYOS
Different factors influencing somatic embryo maturation were studied in olive embryogenic cultures. The effect of sucrose (20, 40, 60, 80 and 100 g L-1), ABA (1, 10 and 100 µM), culture vessel (tubes or petri dishes) and phytagel (3, 6, 9 and 12 g L-1) alone or in combination with 1 g L-1 activated charcoal was analyzed. The influence of each factor was evaluated by quantifying the formation of somatic embryos at different developmental stages and their subsequent germination capacity. The best results were obtained when somatic embryo development was carried out in petri dishes on basal maturation medium with 20 g L-1 sucrose. Under these conditions, significantly higher production of somatic embryos was obtained six weeks after initiation of the maturation phase. Embryos quality appeared not to improve as no significant differences were obtained in terms of germination percentage, number of shoots developed per germinated somatic embryo or shoot length. Higher regeneration potential was obtained under these culture conditions, with 3.75 embryos per culture giving rise to shoot or shoot and root.
Benzekri, H. and Sánchez-Romero, C. (2012). MATURATION OF OLIVE SOMATIC EMBRYOS. Acta Hortic. 961, 441-447
Olea europaea, somatic embryogenesis, abscisic acid, culture container, gelling agent concentration, sucrose, germination