IDENTIFICATION AND EXPRESSION PROFILING OF A LOW OXYGEN REGULATED GENE IN PEACH ROOTSTOCKS USING RT-PCR
The breeding strategy for adaptative traits is based upon the understanding of the physiological and molecular aspects involved in such adaptative response. Waterlogging is an abiotic stress resulting from complete soil inundation and creating a low oxygen (hypoxia) environment in the plants roots. The molecular basis for the adaptation of root tissues to low oxygen conditions in waterlogged soils remains largely unknown. Different studies have shown that fermentation pathways play essential roles for survival under these conditions. Alcoholic fermentation is the most important fermentative pathway for energy production. It is a relatively simple process, where pyruvate is converted into acetaldehyde by pyruvate decarboxylase (PDC), and acetaldehyde is subsequently converted into ethanol by alcohol dehydrogenase (ADH). PDC is the first enzyme channelling carbon towards the ethanolic fermentation pathway and is considered to be the rate-limiting step in this pathway. This suggests a key regulatory role for the first enzyme of ethanolic fermentation. A candidate gene approach was used attempting to identify genes which may be responsible for this adaptation. ESTs sequences from the Genomic Rosaceae Database (GRD) and ESTree database were downloaded. After performing analysis with different bioinformatic programs, tentative consensus sequences were obtained by specific oligo design. Results of cloning and characterization of a low oxygen gene PDC differentially expressed and validated by qRT-PCR under hypoxia conditions in the sensitive almond × peach hybrid Felinem and the tolerant genotype myrobalan plum P.2175 are presented.
Amador, M.L. and Rubio-Cabetas, M.J. (2012). IDENTIFICATION AND EXPRESSION PROFILING OF A LOW OXYGEN REGULATED GENE IN PEACH ROOTSTOCKS USING RT-PCR. Acta Hortic. 962, 135-138
abiotic stress, hypoxia, PDC, RACE-PCR, rootstocks, RT-PCR