IN VITRO GERMINATION OF POLLEN GRAINS FOR PRUNUS PERSICA (L.) BATSCH NUCIPERSICA
The aim of this study was to develop a suitable protocol for in vitro pollen grain germination of nectarine cultivars Centenária and Colombina. Five experiments were done to establish the ideal concentrations of sucrose, agar, calcium nitrate and boric acid as well as optimum pH and temperature values for pollen grain germination and initiation of pollen tube development. A culture medium with 6 g L-1 of agar added with 53 to 57 g L-1 of sucrose, without calcium nitrate and boric acid at pH 6.5 and 20°C was optimal for pollen grain germination of Centenária while 26.3°C was better for Colombina. The highest percentage of germinated pollens is obtained within six hours after inoculation for the Centenária and 12 hours for Colombina. The highest length of pollen tube was observed 12 hours after inoculation of the pollen grains.
Pio, R., Chagas, E.A., Barbosa, W., Chagas, P.C., Bettiol Neto, J.E., Neves, A.A., Carvalho, A.S. and Scarpare Filho, J.A. (2012). IN VITRO GERMINATION OF POLLEN GRAINS FOR PRUNUS PERSICA (L.) BATSCH NUCIPERSICA . Acta Hortic. 962, 261-267
palinology, nectarine, tissue culture, culture medium, reagents