ADMINISTRATION OF TOMATO MODIFIES HEPATIC GLUCOSE AND LIPID METABOLISM IN MICE
Many previous reports have shown that the dietary intake of tomatoes (Lycopersicum esculentum) and tomato products is associated with a reduced risk of chronic diseases. Tomatoes or their components, such as a lycopene, may exert their distinct effects via different mechanisms. However, the biochemical pathways involved in these effects are largely unknown. DNA microarray technology has enabled comprehensive analysis of the expression of a large number of genes simultaneously. Therefore, we used DNA microarrays to investigate the effects of administration of tomato to normal mice on gene expression in the liver. Mice were given tomato beverage (TB) or water (Control) ad libitum for 6 weeks. At 3 weeks, there was no significant difference in body weight between each group, but the body weights and the relative liver weight at 6 weeks in the TB group was significantly lower than those in the Control group. Hepatic gene expression was investigated using DNA microarrays. The ingestion of TB up-regulated the expression of 687 genes and down-regulated the expression of 841 genes, respectively (false discovery rate <0.05). These changes in gene expression suggest that TB ingestion promotes glycogen accumulation and stimulates some specific steps in fatty acid oxidation. In addition, it was indicated the possibility that the decreases in body weight or liver weight loss in the TB group could be attributed to the down-regulation of SREPB-1c and the up-regulation of PPAR α. To our knowledge, the present study is the first to define the effects of tomato on the expression of a large number of genes corresponding to biomarkers in normal mice with microarray technology. The results in this study also demonstrate the importance of consuming tomatoes daily to maintain healthy body conditions and/or reduce the risk of chronic diseases (Aizawa et al., 2009).
Aizawa, K. (2013). ADMINISTRATION OF TOMATO MODIFIES HEPATIC GLUCOSE AND LIPID METABOLISM IN MICE. Acta Hortic. 971, 181-188
DNA microarray, tomato, glucose, lipid, metabolism