CHARACTERIZATION OF PEAR (PYRUS COMMUNIS) CULTIVARS FROM LITHUANIA USING MICROSATELLITE MARKERS
The genus Pyrus includes species and cultivars of great genetic diversity. However, genetic resources of Pyrus have not been fully characterised due to widespread incidence of outcrossing among species and a lack of differentiating morphological markers. Therefore, tools for assessment of genetic polymorphism and genotyping are required for breeding and research on pear genetic resources. Microsatellites are the markers of choice in genetics and breeding studies due to their multi-allelic nature, codominant inheritance, high abundance, reproducibility, transferability over genotypes and extensive genome coverage. In this study, microsatellite markers suitable for genotyping local traditional pear cultivars were characterised. The screen included 18 traditional Lithuanian pear cultivars and 27 common pear cultivars adapted to local climatic conditions. Characterization with eight microsatellite loci revealed 94 alleles. The number of alleles per locus ranged from 8 to 18 (mean value 11.7). For the traditional cultivars, 73 polymorphic alleles were identified (6 to 13 per locus). The most polymorphic microsatellite loci in the 45 pear cultivars in this study were identified with EMPc106 and EMPc117 constituting the minimum set of markers required to distinguish all of the traditional cultivars. The analysis of genetic polymorphism of microsatellite loci were used to establish the genetic relationship among the traditional cultivars. Putative links to known pedigrees and sites of origin were assessed.
Lukoseviciute, V., Gelvonauskienė, D., Baniulis, D., Frercks, B., Sasnauskas, A., Stanys, V. and Rugienius, R. (2013). CHARACTERIZATION OF PEAR (PYRUS COMMUNIS) CULTIVARS FROM LITHUANIA USING MICROSATELLITE MARKERS. Acta Hortic. 976, 257-263
SSR markers, genetic polymorphism, traditional cultivars, genotyping