GENETIC MAPPING AND MARKER-ASSISTED SELECTION OF THE GENE CONFERRING SUSCEPTIBILITY TO ALTERNARIA BLOTCH CAUSED BY ALTERNARIA ALTERNATE APPLE PATHOTYPE IN APPLE
Alternaria blotch, caused by the Alternaria alternate apple pathotype, is an important fungal disease that adversely affects apple production. A major gene Alt controls resistance and/or susceptibility to this disease, and the resistant genotype is indicated as alt/alt. In a progeny of 114 F1 individuals derived from reciprocal crosses between Starking Delicious (susceptible, Alt/alt) and Jonathan (resistant, alt/alt), Alt was mapped to the top of linkage group (LG) 11 by integrated mapping. The nearest marker was DR033892, which is an expressed sequence tag (EST)-derived marker transferred from the linkage map of the powdery mildew resistance genes Pl2 and PlMIS located at the top of LG 11 (Gardiner et al., 2007; Chagné et al., 2008) using the comparative genomic approach. DR033892 was converted to a usable marker by designing primers generating a unique PCR product of 429 bp from the Alt-coupled allele. All of the eight susceptible cultivars tested inherited the Alt-coupled allele of DR033892, suggesting that DR033892 can be used in marker-assisted selection (MAS) with crosses between susceptible cultivars that generate a 429-bp fragment and resistant cultivars that do not generate the same 429-bp fragment.
Moriya, S., Terakami, S., Iwanami, H., Haji, T., Okada, K., Yamamoto, T. and Abe, K. (2013). GENETIC MAPPING AND MARKER-ASSISTED SELECTION OF THE GENE CONFERRING SUSCEPTIBILITY TO ALTERNARIA BLOTCH CAUSED BY ALTERNARIA ALTERNATE APPLE PATHOTYPE IN APPLE. Acta Hortic. 976, 555-560
expressed sequence tag, genome scanning approach, Malus × domestica Borkh., resistance breeding, simple sequence repeat