MOLECULAR CHARACTERIZATION OF GENETIC DIVERSITY OF UNDERUTILIZED CROPS: BUCKWHEAT AS AN EXAMPLE
China has many kinds of underutilized crops which are widely distributed and used for various purposes such as healthy food, industrial materials, cash crops and medicines. Buckwheat is one such crop and considered as a healthy food for the people. China has collected and conserved more than 3000 accessions of buckwheat, composed of landraces, cultivars and wild species. Characterization for agro-economic traits of buckwheat accessions was carried out in China and phenotypic diversity was documented. Recognizing the importance of molecular tools in characterizing crop genetic diversity and identifying useful traits from germplasm collections, Bioversity International in cooperation with the national partners in China aim to characterize genetic diversity of buckwheat with molecular tools such as AFLP, ISSR and SSR, in order to understand the nature of genetic diversity of buckwheat and relationships between the groups with different origins. A total of 79 accessions of Tartary buckwheat (F. tartaricum) were assessed with 15 pairs of AFLP primers and 19 ISSR primers. The results showed that AFLP and ISSR markers have produced high polymorphic information content (PIC) values on buckwheat accessions and could be used for investigating the genetic diversity of buckwheat. Both AFLP and ISSR markers showed that the highest genetic diversity was presented in accessions from Yunnan which is considered the centre of origin of buckwheat. Molecular markers could play an important role in assessing genetic diversity, constructing linkage maps and locating useful genes in underutilized crops.
Zongwen Zhang, and Lijuan Zhao, (2013). MOLECULAR CHARACTERIZATION OF GENETIC DIVERSITY OF UNDERUTILIZED CROPS: BUCKWHEAT AS AN EXAMPLE. Acta Hortic. 979, 407-419
AFLP, ISSR, Tartary buckwheat