IN VITRO PROPAGATION OF SAUROPUS ANDROGYNOUS (SWEET SHOOT): THE POTENTIAL OF DIFFERENT REGENERATION PATHWAYS
Sauropus androgynous (sweet shoot) is an underutilized tropical vegetable classified as useful by the United States Department of Agriculture (USDA) due to its high nutritive value, medicinal properties and economic significance. Sweet shoot is cultivated intensively using stem cuttings in Southeast Asia and is notable for its high palatability. In vitro plant regeneration protocols need to be developed in order to overcome the limitations of conventional propagation through seeds and stem cuttings and to ensure sustainable production of sweet shoot with desirable traits. Relatively limited approaches have been tried for in vitro propagation of sweet shoot, owing to problems such as exudation of phenolics from explants, vitrification and somaclonal variation. This paper provides an overview of recent advances in sweet shoot micropropagation. Micropropagation using nodal stem segments with an axillary bud and the understanding of specific culture requirements at different stages have been adequately addressed in several papers. New challenges for the refinement of protocols for more effective shoot multiplication using liquid media have gained importance recently, due to their advantages over solid medium systems. In sweet shoot, there are several reports demonstrating rapid regeneration and multiplication through organogenesis and somatic embryogenesis using leaf explants. The effects of culture environment on callus induction have been examined to gain a better understanding of callus performance and callus morphology. This paper also describes current and developing methods for in vitro root induction and acclimatization of micropropagated plantlets, since both stages are crucial for successful micropropagation protocols.
Wee, S.L., Alderson, P.G. and Yap, W.S.P. (2013). IN VITRO PROPAGATION OF SAUROPUS ANDROGYNOUS (SWEET SHOOT): THE POTENTIAL OF DIFFERENT REGENERATION PATHWAYS. Acta Hortic. 979, 509-515
shoot multiplication, callus induction, somatic embryogenesis, root induction, acclimatization