OBTAINING VIRUS-FREE PLANTING MATERIAL OF PLUM CULTIVARS BY THE IN VITRO METHOD
Plum pox (Sharka disease) belonging to the economically important diseases in Bulgaria, is a limiting factor for spreading and growing a number of plum cultivars with valuable economic and biological characteristics. The use of the in vitro method creates the possibility of obtaining virus-free plants in a short-term period and in large quantities. The aim of the study carried out referred to developing the technological parameters of micropropagation of seven plum cultivars of the groups Pr. Domestica L. and Pr. Salicina Lind. The factors studied were: sterile culture establishment from explants, suitable nutrient media for multiplication and rooting, adapting to in vivo conditions. It was established that the successful disinfection of the source plant material could be achieved by treatment with 8-10% of potassium hypochlorite for 6-10 minutes depending on the studied cultivars. The optimal multiplication rate was observed when growing the microplants in a modified MS medium supplemented with ВАР 0,5-0,7 mg/L. Increasing the cytokinin above 0,7 mg/L caused an increase of the proliferation effect but initiated vitrification. A high percentage of rooting was established when cultivating the microplants in a MS nutrient medium supplemented with a total or 1/4 content of macroelements and ІВА 1,0-1,5 mg/L. The average height of the stem part, the number and length of the formed rootlets depended to a significant degree on the specific cultivar. The optimal percentage of adaptation to ex vitro conditions was obtained when the rooted plants were planted in a mixture of peat and perlite in a glass-and-steel greenhouse.
Kornova, K.M. and Popov, S.K. (2013). OBTAINING VIRUS-FREE PLANTING MATERIAL OF PLUM CULTIVARS BY THE IN VITRO METHOD. Acta Hortic. 981, 507-512
plum cultivars, micropropagation, rooting, adapting to ex vitro conditions