PROMOTING RADISH AND CARROT SEED GERMINATION USING 5-AMINOLEVULINIC ACID EXTRACT FROM RHODOBACTER SPP.
The effect of 5-aminolevulenic acid (ALA) on germination of Chinese radish and carrot seeds was studied. Variety of seeds were soaked in ALA-hydrochloride (commercial ALA) solution at 0.1-10 ppm and in crude ALA extract from Rhodobacter spp. culture (equivalent to ALA concentration of 2.5 ppm) for 12 h and 20 h for Chinese radish and carrot seed, respectively. The percentage of germination of seed for both species was tested at 25 and 35°C. The results show that ALA-hydrochoride solution concentration and crude ALA solution were able to promote Chinese radish seeds germination more than 90% and mean germination time (MGT) was ranging from 2.25-3.35 d at 25°C. In contrast, at 35°C percentage of germination of Chinese radish seed was less than 50% and MGT was ranging from 4.44-4.97 d for all treatments. For carrot seeds, ALA-hydrochloride and crude ALA could stimulate the seeds germination at 25°C more than control (distilled water). However, none of the treatments at 35°C did show carrot seeds germination. The results suggested that the crude ALA solution has the growth stimulating effects comparable to those of pure commercial ALA.
Tripetch, P., Borompichaichartkul, C. and Srzednicki, G. (2013). PROMOTING RADISH AND CARROT SEED GERMINATION USING 5-AMINOLEVULINIC ACID EXTRACT FROM RHODOBACTER SPP. . Acta Hortic. 989, 339-342
5-aminolevulinic acid (ALA), seed germination, Rhodobacter spp.