PHOTOAUTOTROPHIC CULTURE PHASE FOR TISSUE CULTURED DATE PALM PLANTLETS
Micropropagation has been extensively used for the rapid multiplication of elite date palm cultivars worldwide. However, commercial scale plant production using tissue culture technology is limited due to high percentage of plant loss during the ex vitro transfer from in vitro culture. Under in vitro culture, due to semi-photoautotrophic condition, high humidity and low irradiance, in vitro grown plantlets were weak. The in vitro grown plantlets were often characterized by poor photosynthetic efficiency, malfunctioning of stomata and marked decrease in epicuticular wax and due to these characters, the majority of the plantlets died during the greenhouse acclimatization. Therefore, a transitional culture system is necessary in between semi-photoautotrophic culture phase and greenhouse hardening phase to prepare the in vitro grown plantlets ready to tolerate the ex vitro conditions. In order to prepare the in vitro grown date palm plantlets to ex vitro climatic conditions, the authors developed a low cost transitional photoautotrophic culture phase. During this photoautotrophic culture phase, the plantlets synthesis more epicuticular wax, well developed stomata and well developed root system. The in vitro grown date palm plantlets passed through this newly developed low cost transitional photoautotrophic culture phase showed 100% survival during greenhouse hardening and the resulting plantlets were of marketable superior quality. This newly developed low cost photoautotrophic culture phase would be a boost for the commercial tissue culture industries.
Sudhersan, C., Al-Shayji, Y., Jibimanuel, S. and Ashkanani, J. (2013). PHOTOAUTOTROPHIC CULTURE PHASE FOR TISSUE CULTURED DATE PALM PLANTLETS. Acta Hortic. 994, 313-321
micropropagation, in vitro, ex vitro, acclimatization