MICROPROPAGATION IN VITRO OF OPUNTIA FICUS-INDICA IN SOUTHERN MOROCCO

A. El Finti, R. El Boullani, M. Belayadi, N. Ait Aabd, A. El Mousadik
With the aim of large production of plant material, a rapid micropropagation method of prickly pear cactus (Opuntia ficus-indica) was developed. Young cladode explants containing one areole were cultured on Murashige and Skoog (1962) medium (MS) with adenine sulfate (40 mg/L), monosodium phosphate (50 mg/L), sucrose (50 g/L), phytagel (0.3%) and benzyladenine (BA) at 22.2 µM. Best multiplication rates were obtained with BA at 0.5 mg/L than kinetin. To induce rooting, the proliferated shoots were transferred to a medium containing half MS, sucrose (3%), phytagel (0.3%) and 0.5 mg/L of different auxins. In these culture conditions, 100% of plantlets rooted and highest number of root (21.05 and 16.2) was obtained respectively with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The rooted shoots were easily acclimated and transferred to soil.
El Finti, A., El Boullani, R., Belayadi, M., Ait Aabd, N. and El Mousadik, A. (2013). MICROPROPAGATION IN VITRO OF OPUNTIA FICUS-INDICA IN SOUTHERN MOROCCO . Acta Hortic. 995, 93-98
DOI: 10.17660/ActaHortic.2013.995.11
https://doi.org/10.17660/ActaHortic.2013.995.11
prickly pear, Opuntia ficus-indica, in vitro micropropagation, auxins, micropropagated plantlets
English

Acta Horticulturae