ISOLATION OF NOVEL HYPOXIA RESPONSIVE PERSIMMON ADH, PDC AND ERF GENES USING RNA-SEQ

The physiology of deastringency of persimmon has been widely studied, an anaerobic environment made it by converting soluble condensed tannins (SCTs) into an insoluble form. And alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC) played a key role as the structural gene, which had been regulated by transcription factor ERFs. RNA-Seq is a new technology, widely used in gene isolation and expression. In the present study, 43 novel DkADH, DkPDC and DkERF unigenes were isolated using RNA-Seq, among which three ADH genes (unigene758, unigene12916 and unigene18616), and five PDC genes (unigene11558, unigene22704, unigene24763, unigene30806 and unigene8178) were hypoxia-inducible isoforms. Furthermore, expression of four ERF genes (unigene20841, unigene22129, unigene22569 and unigene24195), highly correlated with target genes, were also hypoxia responsive. These results suggested that these genes might be involved in astringency removal in persimmon.