Construction of yeast cDNA library and screening of TTM3 interaction protein in jujube fruit
Zhao Zixuan is a graduate student in fruit tree science at the school of horticulture, Hebei Agricultural University, China. Her research provides an effective material database and basis for studying the molecular mechanism of jujube fruit development regulation. She constructed a cDNA library of jujube fruit yeast. Jujube fruit is rich in nutrients and contains a variety of bioactive substances such as cyclic adenosine monophosphate (cAMP), polysaccharide and vitamin C. The construction of a jujube fruit yeast cDNA library is important. The jujube genome data show that the expression of the TTM3 gene may be related to the efficient synthesis of cAMP in jujube fruit. The yeast cDNA library using pGADT7 as a vector was constructed by gateway technology. The proteins interacting with TTM3 were screened by a yeast two-hybrid. The library quality test results showed that the library capacity was about 1.44 × 107 CFU, the recombination rate was 96%, and the average length of the inserted cDNA fragment was more than 1 kb. The library was of high quality and met the requirements of yeast hybridization test. The pGBKT7-PHYL1 decoy plasmid was constructed and transferred into Y2HGold yeast cells after no self-activation activity was detected. Seven proteins interacting with TTM3 were screened by yeast two-hybrid verification. The construction of the library has important scientific significance for the study of jujube fruit development, and the regulation mechanism of fruit nutrients.
Zhao Zixuan won the ISHS ISHS Young Minds Award for the best poster presentation at the V International Jujube Symposium in China in September 2021.
Zhao Zixuan, Hebei Agricultural University, 289 Lingyusi street, 071001 Baoding, Hebei, China, e-mail: zhaozx0215@163
The article is available in Chronica Horticulturae