Cytogenetic assessment of Lilium longiflorum × L. hansonii revealed by genomic in situ hybridization (GISH)
My name is Mazharul Islam. I am a PhD student at Kyungpook National University, Daegu, Korea. My major research areas are plant genetics and flower breeding. I hope to introduce noble interspecific and intraspecific Lilium hybrids and develop cytogenetic assessment techniques to evaluate newly generated hybrids. The objectives of my study were to investigate chromosome behavior and inter-genomic recombination during meiosis of L. longiflorum × L. hansonii progenies. Interspecific F1 hybrids (LM) introduced through a cut-style method between two diploid Lilium longiflorum and Lilium hansonii were evaluated cytogenetically by genomic in situ hybridization (GISH) technique. However, GISH analysis of F1 interspecific (LM) hybrids showed equal chromosomal contribution from both female Lilium longiflorum (LL) and male Lilium hansonii (MM). Each of the parents contributed 12 chromosomes except in three crosses: two L. longiflorum ‘White Tower’ × L. hansonii (2x-1) and a L. longiflorum ‘Bright Tower’ × L. hansonii (2x-1). Recombinant chromosomes are not usually found in F1 interspecific lily hybrids. Most often, genomic recombination occurs in crosses between two genetically different parents. Chromosome pairing and cross-over normally occur during meiosis in backcross progenies. However, in this study, genome analysis (GISH) of F1 hybrids (L. longiflorum ‘White Tower’ × L. hansonii) showed four recombinant sites, including two M/L and two L/M recombinant chromosomes, which denotes a high genetic relationship between L. longiflorum and L. hansonii. Therefore, recombinant and non-recombinant chromosomes can be found in F1 interspecific Lilium hybrids. Both parents contributed equally though the chromosome numbers in F1 were not always fixed. Molecular cytogenetic assessment in lily breeding, including flow cytometry, especially GISH, has been a useful tool to easily identify genetic recombination.
Mazharul Islam won an ISHS Young Minds Award for the best oral presentation at the XIII International Symposium on Flower Bulbs and Herbaceous Perennials in Korea in May 2019.
Mazharul Islam, 339, College of Agricultural Sciences, Building number 201, Kyungpook National University, Daegu 41566, Republic of Korea, e-mail: firstname.lastname@example.org
The article is available in Chronica Horticulturae