The relationship between nitrate accumulation and chlorophyll content in leafy vegetables
Melisa Kara is a PhD student at the Hatay Mustafa Kemal University, Faculty of Agriculture, Antakya- Hatay, Turkey. She is focusing on nitrate, nitrite accumulation and is examining the relationship between nitrate accumulation and chlorophyll content in leafy vegetables. Parsley (Petroselinum hortense Mill.) is a very important leafy vegetable. Antakya and Iskenderun are key districts in Turkey for leafy vegetable production and consumption. Greater accumulation of nitrates and nitrites in green leafy vegetables has been associated with negative effects on health, e.g. carcinogenic effects. In this study, the relationships between nitrate, nitrite, total chlorophyll, and chlorophyll a and b in parsley were investigated. Every month, for two years, four samples from each district were collected from local markets. Nitrate and nitrite analyses were conducted by using a DAD detector in an HPLC device. Chlorophyll measurements were determined spectrophotometrically. We concluded that there were no health concerns for parsley in either district, according to the acceptable daily intake (ADI) for nitrate and nitrite determined by the World Health Organization (WHO). The relationship between nitrate and nitrite was not significant in plants from either district, however, there was a significant positive correlation between nitrate and total chlorophyll. Significant positive correlations were also found between nitrite and total chlorophyll. In both districts we have calculated the daily intake amount of parsley for a person (60 kg) that could be safely eaten according to the toxic nitrite limits determined by WHO.
Melisa Kara won an ISHS Young Minds Award for the best oral presentation at the International Symposium on Quality and Safety of Horticultural Products at IHC2018 in Turkey in August 2018.
Melisa Kara, Mustafa Kemal University, Samandag Vocational School, Antakya, Turkey, e-mail: firstname.lastname@example.org