Articles
COMMERCIAL MASS CLONAL PROPAGATION OF GRAPEVINE CV. ‘BIDANEH SEFID’: IN VITRO FRAGMENTED SHOOT APEX CULTURE OF AN ELITE GERMPLASM
Article number
829_61
Pages
383 – 388
Language
English
Abstract
With the aim of developing a rapid and reliable commercial technique for mass clonal propagation of pathogen-free plants of Vitis vinifera cv. Bidaneh Sefid, a unique in vitro propagation method using fragmented shoot apices was examined.
Primary leaf-like structures obtained from shoot apices fragmented in liquid culture, were successfully cultured in vitro in MS basal solid medium containing 2.0 mg L-1 BA. Induced shoots were sub-cultured regularly and the plantlets obtained were rooted in hormone-free 1/2 MS medium.
Plantlets were acclimatized and transferred to glasshouse successfully.
This technique has unique potential to produce commercially large numbers of plants through direct organogenesis in a short period of time.
It could also be a cost-effective way to mass produce clones of rare and elite plant germplasm derived from meristem-tip culture or genetic transformation.
Primary leaf-like structures obtained from shoot apices fragmented in liquid culture, were successfully cultured in vitro in MS basal solid medium containing 2.0 mg L-1 BA. Induced shoots were sub-cultured regularly and the plantlets obtained were rooted in hormone-free 1/2 MS medium.
Plantlets were acclimatized and transferred to glasshouse successfully.
This technique has unique potential to produce commercially large numbers of plants through direct organogenesis in a short period of time.
It could also be a cost-effective way to mass produce clones of rare and elite plant germplasm derived from meristem-tip culture or genetic transformation.
Authors
S.A. Salami, A. Ebadi, Z. Zamani
Keywords
Vitis, mass propagation, germplasm, transformation
Online Articles (69)