Articles
CLONAL FIDELITY OF CHRYSANTHEMUM CULTIVARS AFTER LONG TERM MICROPROPAGATION BY STEM SEGMENT CULTURE
Article number
961_25
Pages
211 – 216
Language
English
Abstract
Morphological characteristics of plants of chrysanthemum cultivars with daisy (Reagan Sunny) and spider flower types (Yellow Spider and Cupper Spider) after long-term micropropagation (15 years) are investigated.
Shoot cultures were established in December 1995 in nodal and internodal stem segment culture on Murashige and Skoog medium supplemented with α-naphthaleneacetic acid and benzylaminopurine (0.5 and 1.0 mg/L, respectively). Shoots are induced directly without callus phase, after four weeks.
Multiplication of shoots by stem segment culture of all cultivars was achieved on same media composition through whole period of micropropagation.
New shoots are formed by activation of axillary buds.
Successful shoot rooting (100%) is achieved during end of May 2009 after three weeks of culture on Murashige and Skoog medium without plant regulators.
Rooted chrysanthemum plantlets were planted at field conditions and successfully flowered during October.
The same color, shape and size of flowers were confirmed for spider type cultivars in both regenerants and mother plants.
Some variations in size, color and distribution of flower parts were observed in the daisy type chrysanthemum cultivar.
The results from this study revealed clonally fidelity of spider type of chrysanthemum, which can be multiplied in long term cultures without any visible change in flower morphology.
On the other hand, long term micropropagaton can be a used for good source for obtaining somaclonal variations for daisy type chrysanthemum.
Shoot cultures were established in December 1995 in nodal and internodal stem segment culture on Murashige and Skoog medium supplemented with α-naphthaleneacetic acid and benzylaminopurine (0.5 and 1.0 mg/L, respectively). Shoots are induced directly without callus phase, after four weeks.
Multiplication of shoots by stem segment culture of all cultivars was achieved on same media composition through whole period of micropropagation.
New shoots are formed by activation of axillary buds.
Successful shoot rooting (100%) is achieved during end of May 2009 after three weeks of culture on Murashige and Skoog medium without plant regulators.
Rooted chrysanthemum plantlets were planted at field conditions and successfully flowered during October.
The same color, shape and size of flowers were confirmed for spider type cultivars in both regenerants and mother plants.
Some variations in size, color and distribution of flower parts were observed in the daisy type chrysanthemum cultivar.
The results from this study revealed clonally fidelity of spider type of chrysanthemum, which can be multiplied in long term cultures without any visible change in flower morphology.
On the other hand, long term micropropagaton can be a used for good source for obtaining somaclonal variations for daisy type chrysanthemum.
Authors
S. Jevremović, A. Subotić, D. Miljković, M. Trifunović, M. Petrić, A. Cingel
Keywords
somaclonal variation, flowering, tissue culture, Chrysanthemum morifolium
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