Effect of desiccation on somatic embryo regeneration in date palm (Phoenix dactylifera L.) cultured in vitro
This work studied somatic embryo formation, maturation and germination using partial desiccation treatment in either sealed test tubes or Plantform bioreactor vessels for different numbers of days. After desiccation, embryogenic callus was transferred to a regeneration medium for 16 weeks. During the study it was found that embryogenic callus subjected to desiccation in both test tubes and sterile bioreactor significantly increased somatic embryo formation, and germinated, when compared to sealed test tubes. However, a perfuse numbers of somatic embryos with good synchronization were obtained whit the use of the TIS (temporary immersion system) system, containing 500 mL liquid MS medium, 0.1 mg L‑1 benzylaminopurine, 0.1 mg L‑1 2ip and 0.1 mg L‑1 kinetin, and a high level of sucrose (100 g L‑1).
Ali, A.H. and Welander, M. (2020). Effect of desiccation on somatic embryo regeneration in date palm (Phoenix dactylifera L.) cultured in vitro. Acta Hortic. 1285, 125-130
Plantform bioreactor, callus, somatic embryo, maturation, TIS system, Phoenix dactylifera