Articles
Effect of lemon juice enriched with melatonin (Citrus lemon L.) in the treatment of K14HPV16 mice
Article number
1387_3
Pages
25 – 30
Language
English
Abstract
Lemon is rich of antioxidant properties and health benefits such as reduction of skin oedema or anticarcinogenic activity which is due to its high content of bioactive compounds, for this is highly appreciated by consumers all over the world.
Melatonin can improve and preserve for longer those properties of lemon, and it has also a protective effect.
The aim of this study is to evaluate the effects of oral administration of lemon juice with melatonin on murinometric parameters of transgenic mice carrying human papillomavirus (HPV) type 16 (K14HPV16) genes, which develop identical injuries to those caused by HPV in humans.
First, lemons were treated by immersion with a dose of melatonin 10 mM. The study was authorized by the ORBEA at UTAD and 40 female mice were used.
The lemon drink was freshly prepared every two days and was made available to the animals as drinking water with three different doses for each group respectively.
Thus, mice were divided into eight groups (G1 to G8, n=5): G1 (WT, control), G2 (WT, [1]), G3 (WT [2]), G4 (WT [3]), G5 (HPV16, control) G6 (HPV16, [1]), G7 (HPV16, [2]), G8 (HPV16 [3]). Group 4 and 8 had the highest concentration of melatonin (308 ng 100 mL‑1). For 28 days, body weight, food, and water consumption were recorded.
In general, food consumption increased in all groups, except group 6, group 7, and group 8 regarding the first and last week.
In terms of drink, it increased in all most groups except for group 2. Only comparing values from the first and last week.
At the concentrations used, the lemon juice drink with melatonin did not interfere with the studied variables.
To get better conclusions the effects of the administered drink in these animals, it is necessary to analyse other parameters, such as the liver or kidney function.
Melatonin can improve and preserve for longer those properties of lemon, and it has also a protective effect.
The aim of this study is to evaluate the effects of oral administration of lemon juice with melatonin on murinometric parameters of transgenic mice carrying human papillomavirus (HPV) type 16 (K14HPV16) genes, which develop identical injuries to those caused by HPV in humans.
First, lemons were treated by immersion with a dose of melatonin 10 mM. The study was authorized by the ORBEA at UTAD and 40 female mice were used.
The lemon drink was freshly prepared every two days and was made available to the animals as drinking water with three different doses for each group respectively.
Thus, mice were divided into eight groups (G1 to G8, n=5): G1 (WT, control), G2 (WT, [1]), G3 (WT [2]), G4 (WT [3]), G5 (HPV16, control) G6 (HPV16, [1]), G7 (HPV16, [2]), G8 (HPV16 [3]). Group 4 and 8 had the highest concentration of melatonin (308 ng 100 mL‑1). For 28 days, body weight, food, and water consumption were recorded.
In general, food consumption increased in all groups, except group 6, group 7, and group 8 regarding the first and last week.
In terms of drink, it increased in all most groups except for group 2. Only comparing values from the first and last week.
At the concentrations used, the lemon juice drink with melatonin did not interfere with the studied variables.
To get better conclusions the effects of the administered drink in these animals, it is necessary to analyse other parameters, such as the liver or kidney function.
Publication
Authors
F. Badiche El Hilali, B. Medeiros-Fonseca, P.A. Oliveira, D. Valero
Keywords
lemon, melatonin, valorization, antioxidant, HPV16, cancer
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