Home > IV Asian Horticultural Congress – AHC2023 > Transcription factors involved THCAS promoter in cannabinoid biosynthesis pathway of Cannabis sativa L.

Articles

Transcription factors involved THCAS promoter in cannabinoid biosynthesis pathway of Cannabis sativa L.

Article number

1404_50

Pages

369 – 376

Language

English

Abstract

Cannabinoids are important secondary metabolites found in Cannabis sativa L. that play a crucial medical role in pain relief and mood adjustment.
The main cannabinoid substances in cannabis are delta-9-tetrahydrocannabinolic acid (THCA), and cannabidiolic acid (CBDA). THCA biosynthesis is regulated by the THCA synthase (THCAS) gene which is regulated by the THCAS promoter.
However, a comprehensive understanding regarding the regulation of gene expression by transcription factors (TFs) in cannabis is lacking.
This study identified the TFs involved in regulating the THCAS promoter in Cannabis sativa L. ‘Hang Kra Rog’ by first amplifying the nucleotide sequences in the promoter of the THCAS gene from cannabis plants using the PCR technique.
Subsequently, the THCAS promoter was cloned into the pENTR-5’-TOPO vector for sequencing purposes and then transferred into the R4L1pDEST_HISi vector by the LR reaction for yeast one-hybrid (Y1H) screening to study the interaction between TF proteins from the transcription factor cDNA library and the THCAS promoter within yeast cells.
The TF proteins involved in the THCAS promoter were identified into seven groups: ARID, B3, bZIP, CPP, GeBP, HD, and NAC with further verification of their functions in cannabis plant cells using transient effector-reporter analysis.

Publication

IV Asian Horticultural Congress – AHC2023

Authors

B. Tontiworachai, R. Deewatthanawong, N. Mitsuda, S. Sakamoto, K. Chung, N. Montri

Keywords

Cannabis sativa L., THCAS promoter, transcription factors, yeast one-hybrid, cannabinoid

Full text

https://doi.org/10.17660/ActaHortic.2024.1404.50

Groups involved

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183. Glycine betaine material affects the growth and yield of asparagus in the long-term harvest production system in a semi-forcing culture

F. Satake | K. Okada | Y. Yoshida | W. Sakata | S. Motoki

184. Mounding increases the rate of thick spears of asparagus

K. Suzuki | Y. Ayabe | A. Kato | S. Motoki

185. Investigation of nutrient solution management in hydroponics using organic fertilizer

T. Endoh | M. Takagaki | P. Suwitchyanon | C. Chaturong

186. Effect of different cultivation methods and environments on the growth and yield of Shimonita-Negi

M. Okabe | K. Obokata | T. Hirose | Y. Hashida

187. Predicting corn salad (Valerianella spp.) weight in a plant factory with artificial lighting (PFAL) using K-Nearest Neighbors algorithm (KNN)

S. Dhammayorddee | C. Chanseetis | A. Kato | M. Takagaki | N. Lookin

188. The effect of water and fertilizer types on growth and yield of supan-supan (Neptunia oleracea) cultivated on flooded Ultisol media

H. Susanti | J. Purnomo | O.A. Kumadawara

189. Effect of low sunlight and high temperature during flower bud differentiation on the cut flower quality of carnation ‘Cherry Tessino’

S. Kamachi | R. Sakai | S. Nagashima | S. Mori

190. Deficit irrigation and fertilizer rates affecting growth, flower quality and physiological changes in tuberous begonia grown in plant factory

C. Inkham | K. Panjama | S. Ruamrungsri

191. Growth and flowering characteristics of Calendula under different light spectra in a controlled environment

M. Munyanont | N. Lu | T. Joilek | D.F. Rachma | M. Takagaki

192. Selection of optimal marigold (Tagetes patula) cultivar as an edible flower in a plant factory

N.Y. Kwak | B.H. Sung | K.P.S. Kumaratenna | Y.Y. Cho

193. Promoting flower yields, photosynthetic pigments and some secondary metabolite in Marguerite daisy (Chrysanthemum frutescens L.) cut flower under the smart farming system by pinching technique

Y. Luekhampa | C. Seelatlao | W. Chareontham | J. Sanitchon | P. Hongpakdee

194. Nutrient deficiencies affect growth and development of vinca

S. Ruamrungsri | C. Inkham | K. Panjama

195. Relationship between Asian ginseng decline and soil chemical property

Md.A.A. Mahmud | Y. Matsubara | T. Ikezawa

196. Influence of different flower vases on the impression of cut flowers

Y. Mitarai

197. Effects of different LED light spectrum on growth and cannabinoid contents of Cannabis sativa L. hybrid ‘KKU’ cultivars under an indoor pot cultivation

P. Nounbunma | P. Hongpakdee | J. Paluka | C. Lapjit

198. Effects of cultivation environmental factors on the growth of the edible cactus (Nopalea cochenillifera) grown under hydroponic conditions

S. Tsuchimoto | K. Okada | K. Kondo | T. Horibe

199. Calcium and magnesium biofortification of the edible cactus Nopalea cochenillifera grown under hydroponic conditions

E. Nishimura | K. Hayashi | T. Horibe

200. Effect of selling area, period, and type on the price of okra (Abelmoschus esculentus L.) in Japan

L. Sato | N. Miyamoto | S. Motoki

201. Comparative analysis of asparagus marketing practices in Europe, North America, and Asia during summer and autumn

S. Yamashita | S. Motoki

202. Edible alleys in the discourse of urban horticulture: a case study on densely built urban renewal districts in Chiayi, Taiwan

T.I. Lee

203. Application of calcium chloride on yield and quality of Capsicum annuum L.

N. Montri | S. Sengseng | R. Deewatthanawong | K. Bunya-atichart

204. Development of a growth state estimation system using a moving camera in strawberry cultivation

S. Matsuo | H. Umeda

205. The impact of adding nanoparticles to the media to speed up the in vitro multiplication stage of banana explants

P. Nugrahani | I.R. Moeljani