Articles
Cell culture induction in three mulberry (Morus alba L.) cultivars
Article number
1422_6
Pages
45 – 50
Language
English
Abstract
Mulberry (Morus alba L.) contains numerous bioactive compounds with antioxidant and anti-inflammatory capacity that can be extracted from the plant.
In vitro cell cultures of mulberry have proven to be a continuous source of polyphenols, in contrast with the discontinuous availability that can be found in leaves obtained from trees.
In this study, three mulberry cultivars (‘Italiana’, ‘Valenciana Temprana’, and ‘Filipina’), previously selected on the basis of their high capacity to produce phenolic compounds, have been used to induce callus formation in order to perform cell cultures.
Small leaves of these three cultivars were disinfected and in vitro cultured to define a protocol for mulberry leaf-cell production.
Media consisting of woody plant medium (WPM) or Murashige and Skoog (MS) medium supplemented with 1 or 2 mg L‑1 of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg L‑1 of kinetin (KN) were tested.
Three different parts of mulberry leaves (leaf, nerve, and petiole) were used to induce callus formation.
After culture, the percentage of callus induction, callus relative growth (CRG), and the percentage of each type of explant that induced callus were recorded.
Overall, in terms of callus induction in mulberry, efficiency was higher using WPM than MS, with a concentration of 1 mg L‑1 of 2,4-D rather than with 2 mg L‑1. In terms of CRG, the concentration of 2,4-D was more significant than the composition of the culture medium, and, on the whole, the highest CRG was obtained with 1 mg L‑1 of 2,4-D. The type of explant that induced a larger percentage of callus was different depending on the culture medium, the genotype, and the concentration of 2,4-D.
In vitro cell cultures of mulberry have proven to be a continuous source of polyphenols, in contrast with the discontinuous availability that can be found in leaves obtained from trees.
In this study, three mulberry cultivars (‘Italiana’, ‘Valenciana Temprana’, and ‘Filipina’), previously selected on the basis of their high capacity to produce phenolic compounds, have been used to induce callus formation in order to perform cell cultures.
Small leaves of these three cultivars were disinfected and in vitro cultured to define a protocol for mulberry leaf-cell production.
Media consisting of woody plant medium (WPM) or Murashige and Skoog (MS) medium supplemented with 1 or 2 mg L‑1 of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg L‑1 of kinetin (KN) were tested.
Three different parts of mulberry leaves (leaf, nerve, and petiole) were used to induce callus formation.
After culture, the percentage of callus induction, callus relative growth (CRG), and the percentage of each type of explant that induced callus were recorded.
Overall, in terms of callus induction in mulberry, efficiency was higher using WPM than MS, with a concentration of 1 mg L‑1 of 2,4-D rather than with 2 mg L‑1. In terms of CRG, the concentration of 2,4-D was more significant than the composition of the culture medium, and, on the whole, the highest CRG was obtained with 1 mg L‑1 of 2,4-D. The type of explant that induced a larger percentage of callus was different depending on the culture medium, the genotype, and the concentration of 2,4-D.
Publication
Authors
A. Gil-Martínez, F.J. Vidal-Sánchez, M. Romero-Muñoz, M. Pérez-Jiménez
Keywords
plant tissue culture, callus production, in vitro culture, kinetin, 2,4-D
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