IN VITRO CULTURE OF RASPBERRY (RUBUS IDAEUS) FOR MASS PROPAGATION AND VIRUS ELIMINATION

M. Welander
A method for large-scale propagation of the cultivar Veten was elaborated which was tested on the following five cultivars : Amber, Mitra, Miranda, Malling Orion and Glen Clova. As Mitra and Miranda were virus infected the method was also used for virus eradication. Growth of meristems was improved if the concentration of NH4NO3 and of KNO3 in Murashige and Skoog (MS) medium was reduced to half strength in combination with 0.2 mg/l BAP and 0.01 mg/l IBA. Sufficient number of axillary shoots were produced, using a slightly modified MS medium supplemented with 1.0 mg/l BAP, provided both shoot tips and nodal segments were used. The number of axillary buds formed from only the shoot tips was very low and it was not possible to increase proliferation rate by changing type and concentration of cytokinins. Addition of different concentrations of GA3 (1, 3 and 5 mg/l) in order to elongate shoots before rooting did not work.

GA3 had little effect on stem elongation and inhibited subsequent rooting. Using a rooting medium with the concentration of the macro-nutrients (MS) reduced to 1 fifth plus 0.01 mg/l IBA and dark treatments for 5 days resulted in 100 percent rooting for four cultivars. Rooted plants survived after being transferred to soil to ≥ 95 percent. Two of the cultivars rooted to less than 10 percent under the same conditions. On the other hand these cultivars produced roots after being transferred to soil and survived to about 90 percent. Plants from eight meristems of Mitra and Miranda were tested for viruses. Viruses could be detected in plants from only one meristem.

Welander, M. (1987). IN VITRO CULTURE OF RASPBERRY (RUBUS IDAEUS) FOR MASS PROPAGATION AND VIRUS ELIMINATION. Acta Hortic. 212, 610-610
DOI: 10.17660/ActaHortic.1987.212.101
https://doi.org/10.17660/ActaHortic.1987.212.101

Acta Horticulturae