Home > Eucarpia Symposium on Fruit Breeding and Genetics > GENETIC TRANSFORMATION OF APPLE (MALUS PUMILA MILL.) FOR INCREASED FUNGAL RESISTANCE: IN VITRO ANTIFUNGAL ACTIVITY IN PROTEIN EXTRACTS OF TRANSGENIC APPLE EXPRESSING RS-AFP2 OR ACE-AMP1

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GENETIC TRANSFORMATION OF APPLE (MALUS PUMILA MILL.) FOR INCREASED FUNGAL RESISTANCE: IN VITRO ANTIFUNGAL ACTIVITY IN PROTEIN EXTRACTS OF TRANSGENIC APPLE EXPRESSING RS-AFP2 OR ACE-AMP1

Article number

484_96

Pages

565 – 570

Language

Abstract

Our optimised protocol for Agrobacterium-mediated transformation of the apple cultivar ‘Jonagold’ was used to transfer genes encoding antimicrobial peptides into this cultivar.
The transgenes encoded either Rs-AFP2, an antifungal peptide from radish, or Ace-AMP1, an antimicrobial peptide from onion, and were placed under the control of the enhanced Cauliflower Mosaic Virus 35S RNA promoter.
Western or dot blot analysis and detection of the antimicrobial peptides in protein extracts of transgenic shoots revealed that the expression level reached a maximum of 0.6% of the total soluble protein content.
After purification over a reversed phase column, protein extracts of Rs-AFP2 expressing shoots showed an eight- to 32-fold increased fungal growth inhibition activity compared to extracts of control plants.
Extracts of Ace-AMP1 expressing plants showed a four-fold increased antifungal activity relative to control plants.

Publication

Eucarpia Symposium on Fruit Breeding and Genetics

Authors

A. Bondt, S. Zaman, W. Broekaert, B. Cammue, J. Keulemans

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1998.484.96

Online Articles (102)

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3. EVALUATION OF FRUIT TREE GENETIC RESOURCES AS SOURCES OF POLYGENIC SCAB RESISTANCE IN AN APPLE BREEDING PROGRAMME

M. Lateur | C. Wagemans | C. Populer

4. VARIATION FOR SUSCEPTIBILITY TO CROWN ROT AND POWDERY MILDEW IN WILD STRAWBERRY FROM NORTH AMERICA

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E. Pauwels | R. Eyssen | J. Keulemans

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9. DEVELOPMENT OF BASE AND ACTIVE COLLECTIONS OF MALUS GERMPLASM WITH CRYOPRESERVED DORMANT BUDS

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11. EX SITU AND IN SITU CONSERVATION STRATEGIES FOR WILD MALUS GERMPLASM IN KAZAKHSTAN

12. LATVIAN APPLE GENETIC RESOURCES AND THEIR POTENTIAL FOR BREEDING

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15. INCREASING GENETIC DIVERSITY FOR APPLE BREEDING: A PRELIMINARY REPORT

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17. INFORMATION SYSTEM ON GERMPLASM OF SWEET CHERRY

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18. SEED DORMANCY AND BUD BREAK OF APPLE SEEDLINGS

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19. GENETIC RESOURCES OF CHERRY PLUM (PRUNUS CERASIFERA EHRH.)

20. SELF-THINNING ABILITY IN JAPANESE PEAR

T. Saito | Y. Sato | K. Abe | O. Terai | K. Kotobuki

21. NEW CHALLENGES FOR APPLE BREEDING

M. Kellerhals | A. Viviani | M. Goerre | C. Gessler

22. THE NAUMBURG/PILLNITZ PEAR BREEDING PROGRAMME RESULTS

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23. GENETIC VARIATION IN A NEW CROP – DOGROSES (ROSA SECT. CANINAE) FOR COMMERCIAL ROSEHIP PRODUCTION

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25. APPLE BREEDING IN NORWAY

D. Røen

26. BREEDING COMMERCIAL APPLE CULTIVARS IN NEW ZEALAND WITH RESISTANCES TO PESTS AND DISEASES

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27. REVIEW OF THE CURRENT APPLE BREEDING PROGRAMMES IN THE WORLD: OBJECTIVES FOR SCION CULTIVAR IMPROVEMENT

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28. NEW APPROACHES TO APPLE SCION BREEDING AT HRI-EAST MALLING

Kate M. Evans

29. RESULTS OF THE SWEET CHERRY BREEDING PROGRAMME IN HUNGARY

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30. APRICOT BREEDING AT THE C.S.I.C. IN MURCIA, SPAIN

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31. PILLNITZER SUPPORTER 4 (PI 80) – A SEMI-DWARF APPLE ROOTSTOCK FROM DRESDEN-PILLNITZ

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32. TREE FRUIT BREEDING PROGRAMMES AT HARROW, ONTARIO, CANADA: PROGRESS 1960–1995

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33. EVALUATION OF BLACK CHOKEBERRY, ARONIA MELANOCARPA, GERMPLASM FOR PRODUCTION OF NATURAL FOOD COLOURANTS

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34. BREEDING STONE FRUIT ROOTSTOCKS IN HUNGARY

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35. SELECTION OF WALNUTS

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36. BREEDING COLUMNAR APPLES IN NOVI SAD

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37. BREEDING CHAENOMELES – A NEW AROMATIC FRUIT CROP

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38. NEW APPLE ROOTSTOCKS, ARM 1, ARM 7 AND ARM 8

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39. BREEDING APPLE CULTIVARS AT THE RESEARCH INSTITUTE OF POMOLOGY AND FLORICULTURE, SKIERNIEWICE, POLAND

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40. PROGRESS IN BLACKCURRANT BREEDING IN ROMANIA

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41. ON THE GENETICS OF INCOMPATIBILITY IN SWEET CHERRIES

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42. REPRODUCTIVE BEHAVIOUR AND INCOMPATIBILITY IN ‘STELLA’ SWEET CHERRY

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43. INHERITANCE OF SELF-COMPATIBILITY IN APRICOT

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44. DISTRIBUTION OF SOME CHARACTERISTICS IN THE SWEET CHERRY PROGENY ‘BIGARREAU BURLAT’ X ‘STELLA’

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45. SELECTION OF SWEET CHERRY SEEDLINGS HOMOZYGOUS FOR SELF-COMPATIBILITY

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46. INHERITANCE STUDIES WITH COLUMNAR TYPE TREES

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49. INHERITANCE OF TREE CHARACTERS IN APPLE

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51. SOME MORPHOLOGICAL TRAITS OF APPLE PROGENIES FROM SCAB-RESISTANT PARENTS

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78. RESISTANCE TO SCAB IN APPLE PROGENIES OF RESISTANT AND SUSCEPTIBLE CULTIVARS

M. G. Tóth | D.X. Quang | S. Kovács | M. Kitley

79. BREEDING FOR HOST RESISTANCE TO PEAR PSYLLA: EVALUATION OF PARENTAL GERMPLASM

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80. MASS SCREENING OF STRAWBERRY SEEDLINGS FOR RESISTANCE TO PHYTOPHTHORA FRAGARIAE HICKMAN

B. Dathe

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82. BREEDING OF PLUM CULTIVARS RESISTANT TO PLUM POX VIRUS

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83. INVESTIGATIONS ON MYCOPLASMA INFESTATION OF SOME PEACH AND NECTARINE CULTIVARS AND HYBRIDS IN ROMANIA

A. Ivascu | V. Balan | G. Solcan | S. Tatu

84. A GREENHOUSE TEST FOR RAPID DETERMINATION OF RESISTANCE TO POWDERY MILDEW IN APPLE VARIETIES AND SELECTIONS

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85. SCREENING OF APPLE CULTIVARS AND SELECTIONS FOR SUSCEPTIBILITY TO THE FUNGAL DISEASES SCAB, MILDEW AND CANKER

H. Kemp | M.C.A. van Dieren

86. CONSIDERATIONS ON SCAB SUSCEPTIBILITY IN APPLE BREEDING

J. Keulemans | K. Claes | A. Brusselle | R. Eyssen | E. Pauwels

87. SELECTION FOR RESISTANCE TO FUNGAL DISEASES IN APPLE

Z.A. Kozlovskaya | T.P. Kurdyuk | G.M. Marudo

88. IDENTIFICATION AND DEVELOPMENT OF MARKERS LINKED TO APHID RESISTANCE IN APPLE

89. BREEDING APPLES FOR SCAB RESISTANCE AT CACAK

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90. ANTHER CULTURE IN APPLE

K. De Witte | S. Schroeven | W. Broothaerts | J. Keulemans

91. EMBRYO RESCUE OF PEACH HYBRIDS

A.B. Küden | E. Tanriver | H. Gülen | S. Büyükalaca

92. ROLE OF FLAVAN-3-OLS IN RESISTANCE OF APPLE TREES TO VENTURIA INAEQUALIS

93. GENETIC ENGINEERING OF APPLE FOR INCREASED RESISTANCE TO FIREBLIGHT

J.L. Norelli | J.Z. Mills | L.A. Jensen | M.T. Momol | H.S. Aldwinckle

94. AGROBACTERIUM-MEDIATED TRANSFORMATION OF THE APPLE CULTIVARS ‘GALA’, ‘GOLDEN DELICIOUS’ AND ‘ELSTAR’, AND THE STRAWBERRY CULTIVARS ‘GARIGUETTE’, ‘POLKA’ AND ‘ELSANTA’

K. Puite | J. Schaart

95. STUDIES ON IMMATURE EMBRYOS IN IN VITRO CULTURE OF INTERSPECIFIC HYBRIDS FROM THE GENUS PRUNUS

V. Balan | A. Ivascu | S. Toma | G. Costea

96. A NEW APPROACH TO OBTAIN POLYPLOID FORMS OF APPLE

Igor V. Bartish | Vitaly I. Korkhovoy | Yelena L. Fomina | Yoong K. Lim

97. GENETIC TRANSFORMATION OF APPLE (MALUS PUMILA MILL.) FOR INCREASED FUNGAL RESISTANCE: IN VITRO ANTIFUNGAL ACTIVITY IN PROTEIN EXTRACTS OF TRANSGENIC APPLE EXPRESSING RS-AFP2 OR ACE-AMP1

A. Bondt | S. Zaman | W. Broekaert | B. Cammue | J. Keulemans

98. ADVANCES IN PROTOPLAST TECHNIQUES FOR MALUS AND PRUNUS

M. Diekmann | V. Hanke | E. Huancaruna-Perales | O. Schieder

99. REGENERATION AND AGROBACTERIUM TRANSFORMATION OF SOUR CHERRY LEAF DISCS

S.V. Dolgov | A.P. Firsov

100. AGROBACTERIAL TRANSFORMATION AND TRANSFER OF THE ANTIFREEZE PROTEIN GENE OF WINTER FLOUNDER TO THE STRAWBERRY

A.P. Firsov | S.V. Dolgov

101. TRANSFORMATION OF RASPBERRY CALLUS BY DIRECT INCORPORATION OF PLASMID PCB1346 AND TRANSFER OF THIS PLASMID INTO APPLE CALLUSES BY COCULTIVATION OF THESE CALLUSES

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102. STUDIES ON GENETIC TRANSFORMATION OF APPLE

A. S. Trifonova | A. I. Atanassov