Articles
A MODIFIED METHOD FOR SIMPLE, RAPID HPLC ANALYSIS OF LYCOPENE ISOMERS
Article number
542_30
Pages
235 – 242
Language
English
Abstract
We describe a rapid method to separate and quantify 
-carotene and geometric isomers of lycopene from tomato fruit.
Carotenoids from extracts of tomato fruit are separated and eluted, using a reversed-phase HPLC with a C30 column and a mobile phase of 40% MTBE, 50% MeOH, and 10% EtOAc.
With this system, we can achieve sharp resolution of cis and trans isomers of lycopene within approximately 25 min instead of the longer, more complex gradient procedures required by previously described methods.
-carotene and geometric isomers of lycopene from tomato fruit.Carotenoids from extracts of tomato fruit are separated and eluted, using a reversed-phase HPLC with a C30 column and a mobile phase of 40% MTBE, 50% MeOH, and 10% EtOAc.
With this system, we can achieve sharp resolution of cis and trans isomers of lycopene within approximately 25 min instead of the longer, more complex gradient procedures required by previously described methods.
Authors
B.K. Ishida, J.C. Ma, B.G. Chan, G.E. Bartley, J.N. Grossman
Keywords
Lycopene, prolycopene, cis-lycopene, HPLC, tomato, tangerine tomato, Lycopersicon esculentum
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