BETALAIN SYNTHESIS BY HAIRY ROOT OF RED BEET CULTURED IN VITRO UNDER DIFFERENT LIGHT QUALITY

H. Boo, K. Shin, J. Heo, J. Jeong, K. Paek
The pigment synthesis of many plants has been usually influenced by various factors including light, temperature, nutrient source, enzyme, growth regulator and/or light quality. The present experiment was conducted to investigate the effect of Light-Emitting Diodes (LEDs) on betalain synthesis in hairy root of red beet (Beta vulgaris L.) cultured in vitro. The hairy roots of red beet, which cultured in the 1/4 liquid MS medium for 14 days were used as a plant material. A bioreactor of 1 L in air volume was used as a culture vessel. The hairy roots were cultured in a growth chamber at controlled air temperature of 25°C and relative humidity of 70%. Blue lights (B), Red lights (R), and the mixing lights of B+R (BR), B+Far-red (BFr), and Red+Far-red (RFr) were used as a light source with a photosynthetic photon flux of 50 μmol m-2 s-1. The growth of hairy root under different light conditions showed a dependence of the biomass formation (fresh and dry weight) on light quality. Sugar concentrations were greatest in hairy roots grown under the R+B and B light, and lowest in hairy roots grown under the R and R+F light. The betacyanin concentration per fresh weight of the hairy root on day 14 was 4.2 times greater in BFr treatment than in R treatment, while their concentration was not significantly different with the B, BR, and fluorescent lamp treatments. Betacyanin/betaxanthin ratio was the highest in BFr treatment and was the lowest in R treatment.
Boo, H., Shin, K., Heo, J., Jeong, J. and Paek, K. (2002). BETALAIN SYNTHESIS BY HAIRY ROOT OF RED BEET CULTURED IN VITRO UNDER DIFFERENT LIGHT QUALITY. Acta Hortic. 580, 209-214
DOI: 10.17660/ActaHortic.2002.580.27
https://doi.org/10.17660/ActaHortic.2002.580.27
Beta vulgaris L., betacyanin, betaxanthin, bioreactor, light-emitting diodes
English

Acta Horticulturae