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Articles

IDENTIFICATION OF ZYGOTIC AND NUCELLAR SEEDLINGS FROM CITRUS ROOTSTOCK CANDIDATES USING RAPD

Article number

738_25

Pages

255 – 260

Language

English

Abstract

The preliminary approach in citrus breeding is to eliminate undesirable genotypes by distinguishing between zygotic and nucellar seedlings.
The identification of true nucellar seedlings with the desired genotype is a daunting process as many citrus varieties produce both nucellar and zygotic embryos.
We have carried out a series of crosses aimed at introgression of mandarin (Citrus reticulata) and pummelo (C. grandis) germplasm to develop alternative rootstocks to sour orange (C. aurantium), and it is necessary to identify those hybrids that yield nucellar seeds for rootstock propagation.
Randomly Amplified Polymorphic DNA (RAPD) marker characterization was used to select hybrids that produce primarily nucellar seedlings.
Five random primers were screened to classify various citrus seedlings from a total of 276 individual progeny obtained from 46 hybrid parental plants.
The degrees of genetic similarity (nucellar) among progeny seedlings for each primer and among all primers were determined and compared with that of the parents.
The usefulness and efficiency of RAPD-PCR method as a quick screening technique for citrus hybrids are discussed.

Publication

International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species

Authors

M. Nageswara Rao, J.R. Soneji, C. Chen, Y.A. Choi, S. Huang, F.G. Gmitter Jr.

Keywords

Citrus reticulata, Citrus grandis, introgression, undesirable genotypes, genetic similarity

Full text

https://doi.org/10.17660/ActaHortic.2007.738.25

Online Articles (105)

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3. RECOVERY OF DIFFICULT-TO-REGENERATE SPECIES: THE CYCAD EXAMPLE

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5. APPLICATIONS OF SOMATIC HYBRIDIZATION AND CYBRIDIZATION IN SCION AND ROOTSTOCK IMPROVEMENT, WITH FOCUS ON CITRUS

6. OVERCOMING CHALLENGES TO DELIVER TRANSGENIC HORTICULTURAL PRODUCTS TO U.S. AND OVERSEAS MARKETS

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7. CAN KNOWLEDGE ABOUT THE MOLECULAR BASES OF PLANT DISEASE AND DISEASE RESISTANCE HELP ENGINEERING RESISTANCE IN CROPS?

O. Le Gall

8. COMMERCIALIZATION OF GENETICALLY ENGINEERED PAPAYA: BREEDING PERSPECTIVES

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9. MOLECULAR GENETICS OF WALNUTS – A BREEDER’S PERSPECTIVE

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10. SITE-SPECIFIC RECOMBINATION FOR PLANT GENETIC ENGINEERING: STRATEGY FOR AGRO-MEDIATED GENE STACKING

D.W. Ow

11. CURRENT CHALLENGES OF TROPICAL TREE CROP IMPROVEMENT: INTEGRATING GENOMICS INTO AN APPLIED CACAO BREEDING PROGRAM

12. USING BIOTECHNOLOGY TO IMPROVE RESISTANCE TO ENVIRONMENTAL STRESS IN FRUIT CROPS: THE IMPORTANCE OF UNDERSTANDING PHYSIOLOGY

M. Wisniewski | C. Bassett | J.L. Norelli | T. Artlip | J. Renaut

13. IS IT POSSIBLE TO INDUCE COLD TOLERANCE IN PAPAYA THROUGH GENETIC TRANSFORMATION?

S.A. Dhekney | R.E. Litz | D. Moraga | A. Yadav

14. INTERGENERIC HYBRIDISATION BETWEEN CARICA PAPAYA AND WILD VASCONCELLEA SPECIES AND IDENTIFICATION OF A PRSV-P RESISTANCE GENE

15. IDENTIFICATION OF CERTAIN PAPAYA CULTIVARS AND SEX IDENTIFICATION IN PAPAYA BY DNA AMPLIFICATION FINGERPRINTING (DAF)

S. Somsri | S. Bussabakornkul

16. CHARACTERISTICS OF RIPE PAPAYA STORED AT LOW TEMPERATURE

C. Wongs-Aree | P. Jitareerat | S. Ketsa

17. PRODUCTION OF TRANSGENIC CITRUS PLANTS USING ISOPENTENYLTRANSFERASE (IPT) POSITIVE SELECTION AND REMOVAL OF THE MARKER GENE BY SITE-SPECIFIC RECOMBINATION

A. Ballester | M. Cervera | L. Peña

18. IDENTIFICATION OF AFLP MARKERS ASSOCIATED WITH CITRUS CANKER RESISTANCE WITHIN CITRUS

Y.A. Choi | C. Chen | S. Huang | F.G. Gmitter Jr.

19. AGROBACTERIUM-MEDIATED TRANSFORMATION IN CITRUS USING A NEW GFP VECTOR

20. SUPPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE (INOS) AND CYCLOOXYGENASE II (COX-2) BY FERMENTED PRODUCT OF CITRUS SUNKI PEEL IN LIPOPOLYSACCHARIDE-ACTIVATED RAW264.7 CELLS

21. MICROARRAY EXPRESSION PROFILING OF NAGAMI KUMQUAT IN RESPONSE TO CANKER

A.A. Khalaf | G.A. Moore | F.G. Gmitter Jr.

22. HIGH-THROUGHPUT APPROACHES TO THE IDENTIFICATION OF CITRUS GENES INVOLVED IN FRUIT RESPONSE TO PENICILLIUM DIGITATUM INFECTION

J.F. Marcos | P. Sánchez-Torres | S. Alamar | L. Zacarías | L. González-Candelas

23. PROTOPLAST CO-TRANSFORMATION AND REGENERATION OF TRANSGENIC ‘HAMLIN’ SWEET ORANGE PLANTS CONTAINING A CDNA XA21 XANTHOMONAS RESISTANCE GENE AND GFP

A.A. Omar | J.W. Grosser

24. A GFP-CONTAINING BINARY VECTOR FOR AGROBACTERIUM TUMEFACIENS-MEDIATED PLANT TRANSFORMATION

V. Orbović | G. Pasquali | J.W. Grosser

25. IDENTIFICATION OF ZYGOTIC AND NUCELLAR SEEDLINGS FROM CITRUS ROOTSTOCK CANDIDATES USING RAPD

26. AGROBACTERIUM-MEDIATED TRANSFORMATION OF CITRUS USING TWO BINARY VECTORS

27. AGROBACTERIUM-MEDIATED TRANSFORMATION OF EMBRYOGENIC CULTURES IN ‘MIYAGAWA WASE’ SATSUMA MANDARIN (CITRUS UNSHIU MARC.)

K.J. Song | S.B. Jin | K.Z. Riu

28. EXPRESSION OF PHI-EA1H PHAGE DEPOLYMERASE GENE WITH CONSTITUTIVE AND INDUCIBLE PROMOTERS, TRANSLATION ENHANCER AND SIGNAL SEQUENCE IN TRANSGENIC APPLE PLANTS INCREASES RESISTANCE TO FIRE BLIGHT

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31. ALTERNATIVE SELECTION SYSTEMS IN APPLE TRANSFORMATION

J. Degenhardt | A. Poppe | L. Rösner | I. Sza

32. MONITORING DIFFERENTIAL EXPRESSION DURING FRUIT MATURATION, RIPENING AND STORAGE AS AN IDENTIFICATION TOOL FOR GENE CANDIDATES FOR SUPERIOR APPLE FRUIT QUALITY

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33. ANALYSIS OF TISSUE UNIFORMITY IN TRANSGENIC APPLE PLANTS

H. Flachowsky | M. Riedel | S. Reim | M.V. Hanke

34. BPMADS4 – A MADS BOX GENE OF BIRCH INDUCES FLOWERS ON TRANSGENIC APPLE PLANTS IN VITRO

H. Flachowsky | M.V. Hanke | A. Elo | T. Sopanen

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M. Höhnle | G. Weber

36. GENETIC TRANSFORMATION OF APPLE WITHOUT USE OF A SELECTABLE MARKER

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M. Malnoy | M. Xu | E.E. Borejsza-Wysocka | S.S. Korban | H.S. Aldwinckle

38. INDUCIBLE DNA PROMOTERS FOR USE IN APPLE

39. IDENTIFICATION AND EFFECT OF TWO BACTERIAL CONTAMINANTS ON APPLE ORGANOGENESIS

M.C. Piagnani | S. Guglielmetti | C. Parini

40. ESTIMATION OF GENE FLOW VIA POLLEN SPREAD FOR THE ORCHARD LAYOUT PRIOR TO THE FIELD RELEASE OF APPLE TRANSFORMANTS

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42. IMPROVEMENT OF ROOTING AND REDUCTION IN PLANT HEIGHT IN APPLE AND PEAR THROUGH GENE TRANSFER

L.H. Zhu | X.Y. Li | M. Nyqvist | M. Welander

43. GENETIC TRANSFORMATION OF APPLE WITH BIBAC VECTORS TO INCREASE SCAB RESISTANCE IN TRANSGENIC LINES

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Y. Kanayama | R. Moriguchi | M. Deguchi | K. Kanahama | S. Yamaki

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G.-Q. Song | R.A. Roggers | K.C. Sink | M. Particka | B. Zandstra

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A. Yamada | R. Tao

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E. de García | C. Villarroel

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69. IDENTIFICATION OF RAPD MARKER LINKED TO RESISTANCE OF MUSA TO FUSARIUM OXYSPORUM

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D.G. Bielenberg | S. Fan | G.L. Reighard | A.G. Abbott

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A. Callahan | R. Scorza

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M. Costa | C. Miguel | M.M. Oliveira

75. IMPROVING REGENERATION AND TRANSFORMATION FOR RESISTANCE TO SHARKA IN PRUNUS

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PH. Druart | R. Gruselle

77. RNA INTERFERENCE FOR SHARKA DISEASE RESISTANCE

78. SUSCEPTIBILITY OF ORNAMENTAL PRUNUS TO PLUM POX POTYVIRUS INFECTION

A. Kalinina | D.C.W. Brown | M. Ravelonandro

79. USING MAT VECTOR SYSTEM TO PRODUCE MARKER-FREE TRANSFORMED APRICOT PLANTS

S. López-Noguera | C. Petri | L. Burgos

80. TRANSGENIC PLUM (PRUNUS DOMESTICA L.) PLANTS OBTAINED BY AGROBACTERIUM-MEDIATED TRANSFORMATION OF LEAF EXPLANTS WITH VARIOUS SELECTIVE AGENTS

R.V. Mikhailov | S.V. Dolgov

81. SETTING UP TISSUE CULTURE TECHNIQUES FOR REGENERATION OF APRICOT TRANSGENIC SHOOTS IN A TRANSFORMATION PROGRAM FOR RESISTANCE TO PLUM POX VIRUS

P. Negri | E. Galassi | S. Tartarini | E. Magnanini

82. MOLECULAR GENETIC DISSECTION OF CHILLING INJURY IN PEACH FRUIT

83. ENDOPOLYGALACTURONASE GENOTYPIC VARIATION IN PRUNUS

84. EFFICIENT TRANSFORMATION OF COMMERCIAL APRICOT CULTIVARS AND REGENERATION OF TRANSFORMED PLANTS

C. Petri | S. López-Noguera | N. Alburquerque | L. Burgos

85. THE EFFECT OF CO-EXISTING PRUNUS VIRUSES ON TRANSGENIC PLUM POX VIRUS RESISTANT PLUMS

M. Ravelonandro | J. Kundu | P. Briard | M. Monsion | R. Scorza

86. CONSTRUCTION OF A GENETIC MAP FOR APRICOT WITH MOLECULAR MARKERS AND IDENTIFICATION OF MARKERS ASSOCIATED WITH PLUM POX VIRUS RESISTANCE

J. Salava | J. Polák | B. Krška | D.A. Lalli | A.G. Abbott

87. ADVENTITIOUS SHOOT REGENERATION IN PRUNUS DULCIS – A MOLECULAR APPROACH TO THE REGENERATION PROCESS

88. DEREGULATION OF PLUM POX RESISTANT TRANSGENIC PLUM ‘HONEYSWEET’

89. ISOLATION OF FULL-LENGTH CDNAS WITH PUTATIVE FUNCTIONS IN ALMOND (PRUNUS DULCIS MILL.) FLOWERING

C. Silva | A.M. Sanchez | M.M. Oliveira

90. TRANSFORMATION OF CHERRY: PRUNUS CERASUS L. ‘MONTMORENCY’ AND PRUNUS CERASUS × P. CANESCEN ‘GISELA 6’ MEDIATED BY AGROBACTERIUM TUMEFACIENS AND A TWO-STEP SELECTION SYSTEM

G.-Q. Song | K.C. Sink

91. THE INFLUENCE OF GENOTYPE ON THE INDUCTION OF SOMATIC EMBRYOS FROM IN VITRO CULTURED ZYGOTIC EMBRYOS AND ADVENTITIOUS SHOOT REGENERATION FROM COTYLEDONS OF PEACH AND NECTARINE

C. Srinivasan | R. Scorza

92. SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM IMMATURE COTYLEDONS OF PRUNUS MUME ‘NANKO’

93. SEROLOGICAL AND MOLECULAR VARIABILITY OF PLUM POX VIRUS IN TRANSGENIC AND CONVENTIONAL PLUMS

94. EXPLOITATION OF STRUCTURAL AND FUNCTIONAL GENOMICS DATABASES FOR GENE IDENTIFICATION IN PEACH

95. GENETIC IMPROVEMENT OF ASEXUALLY PROPAGATED PLANTS

D. Infante | M. Osorio | S. Molina | G. Gonzalez

96. GENETIC DIVERSITY OF VENEZUELAN CASSAVA COLLECTION

97. ROOTING AND ACCLIMATIZATION OF CASSAVA (MANIHOT ESCULENTA) EX VITRO

T.W. Zimmerman | K. Williams | L. Joseph | J. Wiltshire | J.A. Kowalski

98. GENETIC TRANSFORMATION OF EMBRYOGENIC CULTURES AND RECOVERY OF TRANSGENIC PLANTS IN VITIS VINIFERA, VITIS ROTUNDIFOLIA AND VITIS HYBRIDS

99. TRANSGENIC ROOTSTOCK PROTEIN TRANSMISSION IN GRAPEVINES

100. COMPARATIVE ANALYSIS OF STILBENE SYNTHASE GENES AMONG VITIS SPECIES

H. Huang | J. Lu | W. Hunter

101. ISOLATION AND CHARACTERIZATION OF THE 2S ALBUMIN GENE AND PROMOTER FROM GRAPEVINE

102. TOWARDS IDENTIFICATION, ISOLATION AND CHARACTERIZATION OF DISEASE RESISTANT GENES FROM THE NATIVE NORTH AMERICAN GRAPE SPECIES VITIS SHUTTLEWORTHII

J. Lu | H. Huang | Z. Ren | F. Bradeley | W. Hunter

103. ESTABLISHMENT OF EFFICIENT SOMATIC EMBRYOGENESIS AND REGENERATION IN MUSCADINE GRAPE (VITIS ROTUNDIFOLIA)

J. Lu | X. Xu | Y. Jittayasothorn

104. THE EFFECTS OF SALICYLIC ACID ON DISTRIBUTION OF ¹⁴C-ASSIMILATION AND PHOTOSYNTHESIS IN YOUNG GRAPE PLANTS UNDER HEAT STRESS

L.J. Wang | S.H. Li

105. ISOLATION AND CULTURE OF GRAPE PROTOPLASTS FROM EMBRYOGENIC SUSPENSION CULTURES AND LEAVES OF VITIS VINIFERA AND VITIS ROTUNDIFOLIA

X. Xu | J. Lu | D. Dalling | Y. Jittayasothorn | J.W. Grosser