Articles
STANDARDIZATION OF A RAPID SPECTROPHOTOMETRIC METHOD FOR LYCOPENE ANALYSIS
Article number
758_12
Pages
111 – 128
Language
English
Abstract
Health professionals and consumers are becoming increasingly aware of the benefits of lycopene consumption; tomatoes provide the greatest source of this important phytonutrient.
Our laboratory has developed a standard method for the extraction and determination of lycopene in tomato products.
This is a sensitive, rapid and reproducible assay utilizing a mixture of hexane/ethanol/acetone for extraction.
Following incubation for 1 hour, the hexane layer is removed using a Drummond pipettor and lycopene is determined by measuring absorbance at 503 nm.
This method will be useful for breeding programs and processors who are interested in evaluation of a large number of samples in a rapid fashion.
In this manuscript we describe the method and how it was validated.
Our laboratory has developed a standard method for the extraction and determination of lycopene in tomato products.
This is a sensitive, rapid and reproducible assay utilizing a mixture of hexane/ethanol/acetone for extraction.
Following incubation for 1 hour, the hexane layer is removed using a Drummond pipettor and lycopene is determined by measuring absorbance at 503 nm.
This method will be useful for breeding programs and processors who are interested in evaluation of a large number of samples in a rapid fashion.
In this manuscript we describe the method and how it was validated.
Authors
G. Anthon, D.M. Barrett
Keywords
tomato, lycopene, analysis, spectrophotometric
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