A REFINED TISSUE CULTURE MEDIUM FOR IN VITRO PROLIFERATION OF APPLE ROOTSTOCKS
In the present investigation, a protocol for tissue culture of apple rootstocks, using nodal sections from cv. MM111 and cv. MM106, was developed. In order to inhibit the production of phenolic compounds, which are released at the induction stage, combination of ascorbic and citric acid was compared with activated charcoal. The results suggested that the best initiation medium contained full MS medium plus a combination of ascorbic acid and citric acid. The cultures were kept at 4ºC in the dark for six days, and then were transferred to a culture room of 21±1ºC with 16/8 hour light/dark cycle. To optimize multiplication rate, treatments of BAP (0, 1, 2, 4 µM) in combination with GA3 (0, 3, and 6 µM) were applied. Average maximum number of axillary shoots (4.8) and new leaves per regenerated shoots (6) were obtained on the medium containing MS + 2 µM BAP + 3 µM GA3 for MM111. The best multiplication medium for MM106 contained 4 µM BAP, where the average maximum number of axillary shoots (6) and new leaves per explant (5.6) were achieved. When the level of GA3 was increased to 6 µM, maximum increase in stem height for MM111 (20.6 mm) and MM106 (14 mm) were obtained.
Jafarkhani Kermani, M., Hosseini , Z.S. and Habashi, A.A. (2009). A REFINED TISSUE CULTURE MEDIUM FOR IN VITRO PROLIFERATION OF APPLE ROOTSTOCKS . Acta Hortic. 829, 313-318
apple, induction stage, Malling-Merton rootstocks, micropropagation, phenolic compounds