MOLECULAR BIOLOGY AND PROTEOMICS DURING SOMATIC EMBRYOGENESIS IN DIMOCARPUS LONGAN LOUR.
Somatic embryogenesis system in longan can serve as a model experimental system of woody plants. Since 2000, on the basis of histological and cytological observations, determination of endogenous hormones and polyamines (PAs), the changes of isozymes and proteins by PAGE, IEF and SDS- PAGE analysis during longan somatic embryogenesis proteomics and molecular biology during longan somatic embryogenesis have been carried out by mRNA differential display, homologous cloning, fluorescence quantitative PCR, 2-DE and bio-mass spectrometry (MALDI-TOF or Q-TOF2) technique, to uncover the molecular mechanism of longan somatic embryo- genesis, and provide scientific evidence for regulating longan embryo development.
Lai, Z.X., He, Y., Chen, Y.T., Cai, Y.Q., Lai, C.C., Lin, Y.L., Lin, X.L. and Fang, Z.Z. (2010). MOLECULAR BIOLOGY AND PROTEOMICS DURING SOMATIC EMBRYOGENESIS IN DIMOCARPUS LONGAN LOUR.. Acta Hortic. 863, 95-102
real time quantitative PCR, gene cloning, 2-DE, bio-mass spectrometry