Articles
MICROPROPAGATION AND ESTABLISHMENT OF CYPRIPEDIUM ‘LADY SLIPPER’ ORCHIDS
Article number
988_18
Pages
161 – 166
Language
English
Abstract
Root tip explants from mature, blooming size Cypripedium explanted in Steele-Modified Harvais medium containing potato extract were incubated with the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-benzyladenine (BA) or kinetin but not 2,4-D or BA or kinetin alone, produced callus.
If callus remained in contact with plant growth regulators multiple shoots and/or short roots developed.
In contrast, if calli were reflasked into plant growth regulator-free media, then most calli produced plantlets that phenotypically resembled seed-derived seedlings.
Plantlets resembling normal seedlings established readily following vernalization for 3-5 months whereas plantlets with multiple shoots and short roots grow more slowly with lower survival.
If callus remained in contact with plant growth regulators multiple shoots and/or short roots developed.
In contrast, if calli were reflasked into plant growth regulator-free media, then most calli produced plantlets that phenotypically resembled seed-derived seedlings.
Plantlets resembling normal seedlings established readily following vernalization for 3-5 months whereas plantlets with multiple shoots and short roots grow more slowly with lower survival.
Publication
Authors
R.M. Burch, C.B. Chambers
Keywords
orchid, micropropagation, in vitro culture
Online Articles (20)