DIAGNOSIS OF TOSPOVIRUSES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION

R.J. Weekes, R.A. Mumford, I. Barker, K.R. Wood
Immunocaptured virus or RNA extracted from infected tissue, and primers corresponding to segments of the L genomic RNA, were used to identify tomato spot wilt virus (TSWV) by reverse-transcription polymerase chain reaction (RT-PCR). Primers corresponding to sections of the S genomic RNA species were used to detect impatiens necrotic spot virus (INSV). A primer mixture containing sequences for amplified segments of both the L and S viral genomic RNAs was used to detect and differentiate both TSWV and INSV in the same multiplex reaction mixture by producing a PCR product of characteristic size. A second set of universal primers containing sequences for amplification of a different section of the S RNA was also used to detect all tospoviruses tested.
Weekes, R.J., Mumford, R.A., Barker, I. and Wood, K.R. (1996). DIAGNOSIS OF TOSPOVIRUSES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION. Acta Hortic. 431, 159-166
DOI: 10.17660/ActaHortic.1996.431.12
https://doi.org/10.17660/ActaHortic.1996.431.12
https://doi.org/10.17660/ActaHortic.1996.431.12
431_12
159-166
 

Acta Horticulturae